Flow cytometric analysis of androgen receptor expression in human prostate tumors and benign tissues.
نویسندگان
چکیده
Androgen receptor (AR) plays an important role in growth and hormonal therapy of human prostate tumors. Immunohistochemical analysis of AR expression, a nonquantitative technique, is currently used for screening of receptor expression in prostate tissues. The present report describes a laser flow cytometric method for monitoring AR expression in human cell lines and in archival formalin-fixed paraffin-embedded prostate tissues and tumors. Multiparametric flow analysis can be used for simultaneous detection of other cellular markers (e.g., DNA aneuploidy), and by gated analysis, AR expression in subpopulations of a tumor can be quantitatively determined.
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عنوان ژورنال:
- Clinical cancer research : an official journal of the American Association for Cancer Research
دوره 6 5 شماره
صفحات -
تاریخ انتشار 2000